Evaluation of antibacterial and antifungal activity of various concentrations of Azadirachtaindica (Neem) against human pathogenic bacterial and fungal strains

Ashwini R. Tambe, R.M Pawar, S.B. Deokar

Abstract


Drugs  from  natural  sources  are  used  for  treating  various  diseases  since  ancient  times. From  the  literature  it  is  clear  that  various  type  of  pharmacological  and  biological  activities  are  associated  with  Azadirachta  indica. The  objective  of  study  is  to  evaluate  antibacterial  and  antifungal  activity  of  Neem  (Azadirachta indica)  leaves  extracts  (Ethanol  and  Aqueous)  against  bacterial  strain Escherichia  coli(gram  negative), Staphylococcus  aureus (Gram  positive)  and  fungal  strain  Aspergillus  niger.  Ethanol  and  aqueous  extracts  of  varying  concentrations  such  as  0% , 3% , 6% , 9%  were  prepared  and  tested  against  test  microorganisms  using agar well  diffusion  method.The  values  of  Zone  of  inhibition  were tabulated  according  to  the  concentration  of  the  tested  agent  and  data  was  statistically  analyzed.. The  Zone  of  inhibition  showed  efficiency  of  plant  extract. Neem’s  apparent  ability  to   control  certain  strains  of  this  bacterium  is  been  of  great  importance  to  dairying  in  the nations  where  Neem  is  used. The  results  showed  that  E.coli  showed  highest  antibacterial  activity  of  24mm  Zone  of  inhibition  at 9%  as  compaired  with  activity  against  S.aureus  of  22mm  Zone  of  inhibition  at  9%. Antifungal  activity  against A.niger  showed  highest  Zone  of  inhibition  of  19mm  and  15mm  at  9%  concentration  of  both  aqueous and  ethanol  extracts.The  antibiotics  such  as  gentamicin, penicillin  and  antifungicides  such  as  streptomycin  were  tested  against  human  pathogens  as  positive  control. The  antibiotic   and   antifungal   activity  of   leaves  of  Neem  tree  and  their  utility  in  diseases  have  been  confirmed  experimentally. The  results  therefore  confirm  the  traditional  use  of  Neem  for  its  antimicrobial  properties.

Keywords


Azadirachta indica, Escherichia coli , Staphylococcus aureus , Aspergillus niger, Zone of inhibition (ZOI) , Antibacterial activity , Antifungal activity

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