Characterization of Rhizobium strain isolated from the roots of Aeschynomene americana
The soil bacteria Rhizobium species in association with leguminous plants play vital role in agriculture. The amount of nitrogen fixation by rhizobia depends upon the inoculants rhizobia to fix the nitrogen and the ability of plants to provide energy to rhizobia in the nodules. The main objective of the study is to evaluate the nitrogen fixing capacity and characterization of Rhizobium from Aeschynomene americana. The organism belongs to genus Rhizobium is Gram negative shortrods 0.3-0.9µwide1.0to2.0µ long polar and subpolar flagella, producing extracelluar polysaccharide saline. It was found sensitive to temperature, pH and antibiotics. The Rhizobium species isolated from Aeschynomene americana was found to produce alpha-amylase and cellulase and was identified as Rhizobium massiliae (LT560374) on the basis of 16S rRNA sequencing.
Atlas, R.M. (1983). Handbook of microbiological media, CRL, Press, Florida,USA
Aneja, K.R. (2003).Experiments in microbiology plant pathology and Biotechnology 4th edition, New Age International publishers, New Delhi, India
Arora, N. (1954). Morphological development of root and stem nodule of Aeshynomene indica L. Phyto morphology, 4:211-216
Alzard. D. and E. Duhouy (1919), Development of stem nodule in tropical forage legume Aeshynomene afraspera. J. of Experimental. Botany, 41:1199-1206.
Bradshaw, L.J. (1979) “Laboratory Microbiology” 3rd edition W.B. Soundres company, Philadelpia.
Bergersen, F.J. (1980). Methods of evaluating biological nitrogen fixation John Wiley & Sons Intl, USA.
de oliveira A. N, de oliveira L. A, Andrado J. S, Chagas J.A.F (2007). Rhizobia analyse production using various starchy substances as carbon substrates. Brazilian. J. Microbiol. 38 : 208-216.
Dilworth, M. J, Parker, C.A ( 1969). Development of nitrogen fixing system in legumes.J.Theor. Biol. 25: 208-218.
Gao, J.l, Sun, J. G, Liy,Wang Et,chen, W.X ( 1994). Numerical Taxonomy and DNA reletedness of tropical rihozobia isolated from Haingn Province,china.Intl. J. Systematic Bacteriol. 44:151-158.
Hjnaa, A. (1945) Triple-Sugar Iron medium for the identification of internal group of bacteria J.Bacterol. 49: 516-517
Klingeler, I.J (1918). Modifications of culture media uses in the isolation and differentiation of typhoid,dysentery,and allied bacilli. J.Exp.Med.28: 319-322.
Morales V.M. Martinez - Motina E1,Hubbel DH (2005) Celluase production by Rhizobium plant soil 80: 407-415.
Skinner, F.A. and D.W. Lovelock (1979) Identification method for microbiologist Academic Press, USA.
Subba Rao, N.S. (2006) Soil microbiology 4th edn oxford and IBH publishing Co.Pvt. New Delhi, India.
Vincent, J.M. (1970) A manual for the practical study of root nodule bacteria IBP Handbooks 15 International Biological programme.
Yatazawa, M. and S. Yoshida (1979) Stem nodule in Aeshynomene indica and their capacity of nitrogen fixation. Physiol. Plant, 45:293-295.
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