Protein Profile Studies – Effect of Flavonoids and Glycosides from Caesalpinia Coriaria (JACQ) Willd as Bactericidal Compound.

Anandhi Dhananvel, Praveen Kumar, Jagatheesh .

Abstract


Scope:  The scope of the present study was to identify the bioactive compounds and to investigate the mode of action as antibacterial compound (Protein Profile Studies) from Caesalpinia coriaria (Jacq) Willd.

Materials and Methods: Isolation of flavonoids and glycosides from the ethanolic extract of Caesalpinia coriaria was carried and phytochemicals were analyzed by TLC. Flavonoids and glycosides compounds were treated with E.coli, Staphylococcus aureus and Klebsiella pneumonia resulted in the disturbance in protein synthesis which may act as bactericidal effect of the medicinal plant.  .

Conclusion: Based on the current findings, the action model of these two bioactive compounds results in the disturbance of protein synthesis. Hence the bioactive compounds enter the inner membrane and inactivate protein profile thus inhibiting the growth of cells. Thus the result suggests that Caesalpinia coriaria was a potential candidate plant for the management of organism for antibacterial activity.


Keywords


Antibacterial activity, Caesalpinia coriaria, E.coli, Staphylococcus aureus, Klebsiella pneumonia, flavonoids, glycosides, TLC-Thin Layer Chromatography, SDS- PAGE.

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