Evaluation of antimicrobial activity of probiotic microflora from neonatal origin.

Smita Hemant Nilakhe


The antimicrobial effects of the bacteriocin obtained from nine Lactic Acid Bacteria isolated from the fecal sample of breastfed neonates were tested against three pathogenic indicator strains, Shigella flexineri NCIM5265, Bacillus cereus NCIM2079 and Proteus vulgaris NCIM2027. 20 hrs old cultures supernatant was used to perform the antimicrobial activity using well diffusion method. All the nine isolates showed good inhibition of Shigella flexineri and Bacillus cereus. While seven isolates of nine showed moderate inhibition of Proteus vulgaris .Two isolate showed no inhibition to Proteus vulgaris.


antimicrobial effects,breastfed neonates,well diffusion method

Full Text:



FAO/WHO: Expert consultation on evaluation of health and nutritional properties of probiotics in food including milk powder with live lactic acid bacteria. 2001 [http://www.who.int/ foodsafety/publications/fs_management/en/probiotics.pdf].

Mohammed, S. Balogu, T. Yunusa,A. and Aliyu,H. (2013), Antimicrobial Efficiency of Purified and Characterized Bacteriocins Produced by Lactobacillus bulgaricus Y34 and Lactococcus lactis N22 Isolated from Fermented Milk Products. International Journal of Advanced Research. 1, 6, 63-70

Ogunbanwo, S.T., Sanni, A.J. and Onilude, A.A., (2003), Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1., African Journal of Biotechnology, 7,179-184.

Siamansouri, M. Mozaffari , S. and Fatemeh (Elham) Alikhani ,(2013), Bacteriocins and lactic acid bacteria., Journal of Biology and today's world, 2, 227-234.

Yoshioka, H. Iseki, K.Fujita, K. ,(1983), Development and differences of intestinal flora in the neonatal period in breast-fed and bottle-fed infants, Pediatrics,72,317–21.

Versalovic J ,Michel Wilson, Therapeutic Microbiology, ASM Press. (2008)


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.