The Effects of Astragalus sp/ Euphorbia Cheriradenia Honey Supplementation on acute exhaustive exercise on Lipid Peroxidation of liver after a Bout Acute Exhaustive Treadmill Exercise in Rats

Bijan Goodarzi, Amir Khosravi


The purpose of the present study was to investigate the effects of honey, at a dose (1.5 g/kg weight per d) on the MDA and SOD levels of liver in rats after exhaustive exercise. Male rats (n=48) were divided into four groups, group1, sedentary with distilled water (Sed -DW n=12), group2, sedentary control with honey (Sed-H N=12), group3, exercise trained with distilled water (ET-WD n=12), group4, exercise trained with honey (ET- H n=12) groups. Four groups were further divided equally into two groups where the rats were studied at rest and immediately after exhaustive exercise. During the training period, groups2‚4 were treated with 1.5 g/kg body weight honey was freshly diluted by distilled water to 50% and other groups 1‚3 treated with distilled water (1 ml) just before each administration intragastrically tube daily at 8:00 to rats / once daily for 8 weeks. Endurance training consisted of treadmill running 1.5 h day-1, 5 days a week for 8 weeks, reaching the speed of 2.1 kmh-1 at the fourth week. For acute exhaustive exercise, graded treadmill running was conducted reaching the speed of 2.1 km h-1 at the 95th min, 10% uphill, and was continued until exhaustion. Liver MDA level was significantly increased after exhaustion in groups 1‚3 but not in groups 2‚4 animals compared with the corresponding sedentary rest. Honey treatment caused a significant decrease in MDA levels of groups 2‚ 4 compared the groups 1‚ 3 animals. While acute exhaustive exercise decreased liver SOD activity in subgroups 1‚ 3 rats, it increased the activity of this enzyme in groups 2‚ 4 rats .Treadmill training increased the endurance time in trained rats compared with sedentary rats. The results of this study suggest that honey Supplementation may be useful to prevent acute exhaustive exercise-induced oxidative stress in liver by up-regulating some of the antioxidant enzyme activities and may have implications in exercising humans.


Endurance training ,liver , Exhaustive exercise , SOD , Lipid peroxidation

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